Lab report Example | Topics and Well Written Essays - 1000 words - 5

Lab Report Example In the course of the experiment, the presence of a peptide bond through the application of the method would result to a copper (ii) ion forming violet color in an alkaline solution. The Biuret test in this experiment is used in the determination of the concentration since peptide bonds occur with the same frequency in each amino acid in the peptide. This is possible in this experiment since the absorption at 550 nm is directly proportional to the protein concentration in accordance to Beer-Lambert law, thus enabling the experiment to determine the level of protein concentration in a solution (Janairo et al. 2011). Folin protein assay is a biochemical assay for determination of the total level of protein in a solution. In this experiment, the concentration of protein is manifested in the color change in the sample solution in proportion to the concentration of protein. The method is based on the reaction of Cu+ produced by the oxidation of the peptide bonds (Lowry et al. 1951). For the biuret protein assay, when 3mg protein is used in the experiment, the average absorbance in 550nm is 0.690. For 2.5 mg, the average absorbance is 0,440nm, for 2mg protein, the average absorbance is 0.588nm. When 1.5 mg protein is used, the average absorbance is 0.564 nm, 1mg result to average absorbance of 0.540nm while 0.5ng solution results to an average absorbance of 0.224nm. In tube X the average absorbance is 0.454nm while tube Y which contains approximately half of the volume of X results to an average absorption of 0.232nm. From the experiment, it is evident that the amount of absorption is directly proportionate to the amount of protein concentration in a solution at a standard absorption level of 550nm absorbance. Thus, the biuret protein assay test is a concrete test that can be used in the determination of the peptide bonds and the amount of proteins in a solution (Lipscomb et al. 2006). For